Thin-walled 0.1 mL Individual PCR Tube, with attached Flat cap, Low Profile, Yellow

White PCR products yield significantly better results in qPCR than transparent vessels. In combination with the smooth surfaces, the white color ensures optimal reflection of the fluorescence signals.

When used as instructed, these vessels are remarkably robust and can handle typical laboratory conditions, without fracturing or resulting in loss of volume. It is recommended that, when applying caps, a proper tray be used to protect the bottom of the tubes from excessive force that is typically used.

Yes. All of the UTW plastics we provide are well suited for qPCR reactions.

We recommend low profile PCR plates and tubes for reaction volumes below 20 µL. The low profile design minimizes the airspace between the PCR reaction and reduces evaporation effects, thereby increasing PCR efficiency in low volume reactions.

Low profile PCR plastics have shorter wells than the standard profile plastics, decreasing the “dead space” between the heated lid of the thermal cycler and the sample. This eliminates condensation on the side wall of the tube, preventing reduction in PCR volume and increasing the efficiency of the reaction.

No. You need a flat highly transparent cap to detect the fluorescence signals produced during a quantitative Real Time PCR .

Please select your real-time PCR (qPCR) or thermal cycler (PCR) instrument model from the drop-down menu in the qPCR and PCR Plastics Selection Tool to see the list of all validated plastic consumables (plates, tube strips, single tubes, caps, seals, adhesive films, and accessories) we offer that are compatible with your instrument.

They are not certified endotoxin-free. However, their entire production process, from molding to final packaging, is carried out in a Class 100,000 clean room under ISO 9001 guidelines. All PCR plastics are certified free from RNase, DNase and human DNA.

PCR plastics are not typically sterilized as their entire production process, from molding to final packaging, is carried out in a Class 100,000 clean room under ISO 9001 guidelines. All PCR plastics are certified free from RNase, DNase and human DNA. In contrast, during typical non-clean room production, plastics are exposed to many contaminants including dust, bacterial cells, and DNA. The plastics are then sterilized to kill bacteria and inactivate RNases and DNases, but sterilization does not remove dust or DNA contamination. The dust particles left behind can inhibit PCR, and the damaged DNA fragments can still act as templates, leading to non-specific amplification.

The smaller the sample volumes, the more important it becomes to prevent evaporation loss. Thanks to the large selection of different sealing films and strips, you always have a perfectly harmonized system to securely seal your PCR plates and vessels.

Plates with standard profile wells usually have a nominal volume of 0.2 ml per cavity, for low profile plates 0.15 ml.

Although PCR consumables are made of PP (which in general is autoclavable at 121°C (2 bar, acc. to DIN EN 285) we do not recomment to autoclave these tubes. Mainly, because PCR products have very thin walls and secondly because the autoclave could be a source for contamination.

PCR sealing mats have been designed to fit PCR plates. They can reduce evaporation losses up to 75% compared to conventional systems. They are charcterized by their high flexibility, tight sealing, autoclavability and are easily pierced by pipette tips.

Sealing mats can be autoclaved at +121°C (2 bar, acc. to DIN EN 285). However the silicone sealing mat (Cat. No. 781408) can only be autoclaved ~ 5 x because of material shrinkage.