The hepatic stellate cells (HSCs) localize at the space of Disse in the liver and have multiple functions.
The human intrahepatic biliary epithelium is composed of a morphologically heterogeneous population of epithelial cells.
In this protocol, we will deseribe method for isolation of human hepatic sinusoidal endothelial cells(HHSEC) harvested from adult patients undergoing liver resectionfor metastatic cancers.